Bile Salts And Pancreatic Lipase. This effect is parallel to and probably mediated by a decrease in the SDS monomer concentration as the result of the. Bile salts emulsify the fat and pancreatic lipase hydrolyzes them to release fatty acids and glycerol.
Why do bile salts inhibit pancreatic lipase. The simultaneous presence of bile salts protects the enzyme from being irreversibly inactivated. Bile salts are adsorbed on the interface of triglyceride droplets and hinder the adsorption of pancreatic lipase.
A study using the oil drop tensiometer.
The rate of hydrolysis of long chain triglycerides by pure bovine pancreatic lipase has been determined in the presence of variable amounts of bile salts and colipase. Co-lipase overcomes the inhibition of lipase caused by bile salts. Sodium dodecyl sulfate SDS binds to pancreatic lipase in a cooperative manner up to about 200 molesmole of protein. But it is also said that pancreatic lipase do need a colipase to overcome the inhibitory effect from bile salts.
